Isolation and genetic stability of an infectious bronchitis virus strain (IBV)

Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens

Molecular Cloning and Expression of Nucleocapsid Gene of Chicken Infectious Bronchitis Virus Strain Massachusetts H120

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

Comparative anti-infectious bronchitis virus (IBV) activity of (-)-pinene: effect on nucleocapsid (N) protein.

In the present study, anti-IBV (infectious bronchitis virus) activities of (-)-pinenes were studied by MTT assay, as well as docking and molecular dynamic (MD) simulations. The CC₅₀ values of (-)-α-pinene and (-)-β-pinene were above 10 mM. And the maximum noncytotoxic concentrations (TD₀) of (-)-α-pinene and (-)-β-pinene were determined as 7.88 ± 0.06 and 6.09 ± 0.31 mM, respectively. The two c...

isolation and identification of infectious bronchitis virus from commercial chickens

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Genetic Analysis of Three Structural Proteins in Iranian Infectious Bronchitis Virus Isolate

Infectious bronchitis virus (IBV) is a contagious pathogen in fowl that results in economic loss in the poultry industry. In this study, the amino acids sequences of three structural proteins M, N, and S1 for five Iranian IBV isolated during 1998-2011 have been analyzed. Conserved and variable regions, hydrophobic characteristics and identity matrix were determined after alignment by Bioedit ve...